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Tissue preservation

Pancreas Preservation by Two-Layer Method

Hypothermic preservation is one of the main-stream organ preservation methods. The Two-Layer Method (TLM) of organ preservation is found to be the most effective of all available hypothermic cold storage methods. The objective of this work is to test the hypothesis that the putative functional efficiency of the TLM is due to the favorable heat transfer effect between pancreas tissues and the two solutions viz. the University of Wisconsin (UW) solution and Perfluorocarbon (PFC) solution. In this research, both experimental and numerical approaches are undertaken to study the heat transfer phenomena involved.

The numerical analysis of the problem involves natural convection within two phases of the immiscible fluids namely the UW and PFC solutions and hence a multiphase model is employed. The experimental study deals with measuring the cooling rates of a pancreatic tissue preserved in the following fluids: 1) UW solution only 2) PFC solution only 3) TLM comprising of UW and PFC solutions.  tissue1.jpg                                                                                         

Fig 1. (A) TLM experimental setup (B) Velocity vector at 5 min of tissue surrogate preservation (C) 2D Axi-symmetric model used for numerical computation


A 2D axi-symmetric model of the TLM with a pancreas tissue surrogate and container modeled as co-axial cylinders is as shown in Fig 1C. A multi-phase heat transfer model is employed to solve for natural convection. The velocity vectors near interface section at the 5th minute of preservation obtained by numerical computation are as shown in Fig 1B.


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by zopeown last modified 2015-05-26 12:01